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Stem Cells International
Volume 2017 (2017), Article ID 3794817, 11 pages
https://doi.org/10.1155/2017/3794817
Research Article

Transplantation of Rat Mesenchymal Stem Cells Overexpressing Hypoxia-Inducible Factor 2α Improves Blood Perfusion and Arteriogenesis in a Rat Hindlimb Ischemia Model

1Department of Vascular Surgery, Zhongshan Hospital of Xiamen University, Xiamen, China
2Cancer Research Center, Medical College of Xiamen University, Xiamen, China
3Department of Cardiovascular Surgery, Xin Hua Hospital affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, China
4Department of Vascular Surgery, Zhongshan Hospital of Fudan University, Shanghai, China

Correspondence should be addressed to Weifeng Lu

Received 3 January 2017; Accepted 29 August 2017; Published 7 November 2017

Academic Editor: Boon C. Heng

Copyright © 2017 Weifeng Lu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplementary Figure 1 Verification of the expression of HIF-2α in recombinant lentivirus. A. Image of agarose gel showing successful insertion of HIF-2α gene fragment in the vector. The insertion was confirmed by DNA sequencing of the recombinant vectors. The markers (2nd column) were 5 kb, 3 kb, 2 kb, 1.5 kb, 1 kb, 750 bp, 500 bp, 250 bp, 100 bp from top to bottom respectively. B. A representative image of Western blot showing that HIF-2α was expressed successfully. 293 cells transduced with recombinant lentivirus over-expressing HIF-2α or with empty lentivirus were harvest. Total proteins were extracted, separated on SDS-PAGE gel, and transferred to a PVDF membrane. The membrane was probed with anti-FLAG antibody. Lane 1 is the sample from 293 cells transduced with empty lentivirus and lance 3 is the sample from 293 cells transduced with the recombinant lentivirus. C. A representative fluorescence image showing 293 cells transduced with recombinant lentivirus. D. RT-PCR verification of HIF-2α expression in the 293 cells transduced with the recombinant lentivirus RT-PCT. The mRNA level of HIF-2α was dramatically increased in 293 cells transduced with the recombinant lentivirus compared to the controls.

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