Long-Term Safety of Transplanting Human Bone Marrow Stromal Cells into the Extravascular Spaces of the Choroid of Rabbits

Figure 1

Identification of transplanted hBMSCs in the EVSC compartment 2 hours following transplantation. Frozen sections of rabbit eyes removed 2 hours following transplantation of nonlabeled hBMSCs were stained with anti-human nuclei antibody (red, (h) and (i)) to identify the transplanted human cells. White arrows highlight transplanted cell. Sections counterstained with DAPI only (no antibodies, (a), (b), and (c)) or incubated with secondary antibody only (d), (e), and (f) are presented as control. All sections were counterstained with DAPI (blue). CH: choroid; SC: sclera; Ab: antibody. Scale bar 100 μm.