Figure 4: Transplanted hBMSCs are located between the blood vessels in the EVSC. Cross sections of eyes removed 4 days following transplantation of Dil-labeled hBMSCs (red) were stained with an antibody directed against smooth muscle actin (green, (b) and (c)) and photographed using confocal microscopy. (a) The section was incubated without a primary antibody. (b) Dil-labeled cells are clearly identified across the ECVS. The white asterisk highlights red blood cells inside a blood vessel. Scale bar 25 μm. (c) A representative confocal image with orthogonal views (c1 and c2) demonstrating the absence of Dil-labeled cells inside the blood vessel.