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Stem Cells International
Volume 2017 (2017), Article ID 4061975, 13 pages
Research Article

Long-Term Safety of Transplanting Human Bone Marrow Stromal Cells into the Extravascular Spaces of the Choroid of Rabbits

1Maurice and Gabriela Goldschleger Eye Institute, Sheba Medical Center, Tel Hashomer, Israel
2Sackler School of Medicine, Tel Aviv University, Tel Aviv, Israel
3Center for Stem Cells and Regenerative Medicine, Sheba Medical Center, Tel Hashomer, Israel
4Department of Chemistry, Bar-Ilan Institute of Nanotechnology and Advanced Materials, Ramat Gan 52900, Israel
5Hematology Division, Sheba Medical Center, Tel Hashomer, Israel

Correspondence should be addressed to Ygal Rotenstreich

Received 1 November 2016; Revised 26 February 2017; Accepted 2 April 2017; Published 18 June 2017

Academic Editor: Yonathan Garfias

Copyright © 2017 Adi Tzameret et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplementary Figure 1. Identification of hBMSCs in the EVSC 2 weeks following transplantation. Frozen sections of rabbit eyes removed at 2 weeks following transplantation of hBMSCs were stained with DAPI only (with no antibodies, panels A-C) or incubated with secondary antibody only (D-F). All sections were counter-stained with DAPI (blue). The lack of nuclear staining in the EVSC demonstrates the specific staining of the anti human nuclei antibody shown in Figure 2. Scale bar 100µm. Ab- antibody. Supplementary Figure 2. Transplanted hBMSCs are located between the blood vessels in the EVSC compartment. Frozen sections of eyes removed 4 days following transplantation of DiI-labeled hBMSCs (red, B & D) were stained with an antibody directed against smooth muscle actin (SMA, green, C & D) and photographed using a fluorescent microscope. Sections were counter-stained with DAPI (blue, A &D). Scale bar 100 µm. Supplementary Figure 3. Representative fluorescent images of a non-transplanted rabbit eye demonstrating the tissue autofluorescence. Frozen sections of non-transplanted eyes were stained with DAPI and photographed with a fluorescent microscope using a similar exposure time (200 msec) used for photographing the anti-human nuclei antibody staining of transplanted eyes. ONL – outer nuclear layer; OS – outer segments; CH- choroid; SC-sclera. Scale bar 100 µm.

  1. Supplementary Material