Canine Adipose Derived Mesenchymal Stem Cells Transcriptome Composition Alterations: A Step towards Standardizing Therapeutic

Table 1

Antibody panel used for flow cytometry analysis.

Cell surface marker	Antibody clone	Species reactivity	Host	Clonality	Source	Expression on cASCs
CD14 PE-Cy7	M5E2	Canine, human	Mouse	Monoclonal	BD Pharmingen	Negative marker
CD44 FITC	YKIX337.8	Canine	Canine	Monoclonal	eBioscience	Positive marker
CD45 PE	YKIX716.13	Canine	Rat	Monoclonal	AbD Serotec	Negative marker
CD90 APC	YKIX337.217	Canine	Canine	Monoclonal	eBioscience	Positive marker
CD271 FITC	ME20.4-1.H4	Canine	Mouse	Monoclonal	Miltenyi Biotec	Positive marker
CD 29 FITC	MEM-101A	Human, canine, pig	Mouse	Monoclonal	Antibodies-online.com	Positive marker
IgG2a PE-Cy7	G155–178		Mouse	Monoclonal	BD Pharmingen
IgG2a FITC	eBR2a		Rat	Monoclonal	eBioscience
IgG2b PE	R35–38		Rat	Monoclonal	BD Pharmingen
IgG2b APC	eB149/10H5		Rat	Monoclonal	eBioscience

CD: cluster of differentiation, Ig: immunoglobulin, APC: allophycocyanin, PE: Phycoerythrin, FITC: fluorescein isothiocyanate, PE-Cy7: R-Phycoerythrin-Cyanine 7.