Research Article

Ex Vivo Expansion of Human Limbal Epithelial Cells Using Human Placenta-Derived and Umbilical Cord-Derived Mesenchymal Stem Cells

Figure 2

Coculture of MSCs and limbal epithelial cells (LECs) and colony-forming unit assay. Schematic of the coculture system containing LECs (blue in the insert) and MSCs (not shown). Yellow indicates medium. (a) Microscopic analysis following H&E staining (b; magnification: ×10) and immunofluorescence of p63 and ABCG2 (c; white bar indicates 500 μm) are shown. Error bars represent the standard deviation of the mean. , compared with the control medium, CnT-PR (Student’s t-test). C-MSC: umbilical cord-derived mesenchymal stem cell; P-MSC: placenta-derived mesenchymal stem cell; ABCG2: ATP-binding cassette subfamily G member 2.
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