Long Noncoding RNA uc001pwg.1 Is Downregulated in Neointima in Arteriovenous Fistulas and Mediates the Function of Endothelial Cells Derived from Pluripotent Stem Cells

<div>Characterization and differentiation of endothelial cells (ECs) derived from human induced pluripotent stem cells (HiPSCs). (a) FACS analysis showing the CD31 positive cells population upon differentiation protocol. FACS sorting by the endothelial markers CD31 at day 10. Efficiency to generate CD31-positive cells was 16.8%. (b) Differentiation of HiPS toward ECs. A phase-contrast image of cell appearance at 0 day (top left panel), 3 days (top right panel), 7 days (bottom left panel), and 10 days (bottom right panel) after differentiation. Scale bar = 50 <i>μ</i>m. (c) Immunofluorescent images of CD 31-positive HiPSC-ECs on day 10 during EC differentiation. DAPI was used and stained the cell nucleus. Scale bar = 50 <i>μ</i>m.</div>

Stem Cells International

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Figure 2: Long Noncoding RNA uc001pwg.1 Is Downregulated in Neointima in Arteriovenous Fistulas and Mediates the Function of Endothelial Cells Derived from Pluripotent Stem Cells 

Figure 2 | Long Noncoding RNA uc001pwg.1 Is Downregulated in Neointima in Arteriovenous Fistulas and Mediates the Function of Endothelial Cells Derived from Pluripotent Stem Cells 