Research Article
Endometritis and In Vitro PGE2 Challenge Modify Properties of Cattle Endometrial Mesenchymal Stem Cells and Their Transcriptomic Profile
Table 1
Details of primers used for qRT-PCR analysis of selected genes used in the PCR experiments.
| | Gene name | Primer sequences | Annealing T (°C) | GenBank accession number |
| | OCT4 | F: 5-GGAGAGCATGTTCCTGCAGTGC 3
R: 5-ACACTCGGACCACGTCCTTCTC 3 | 58°C | NM_174580 | | NANOG | F: 5-TTCCCTCCTCCATGGATCTG 3
R: 5-ATTTGCTGGAGACTGAGGTA 3 | 58°C | NM_001025344 | | SOX2 | F: 5-CGAGTGGAAACTTTTGTCCG 3
R: 5-GGTATTTATAATCCGGGTGTT 3 | 55°C | NM_001105463 | | CD44 | F: 5-GACTGTACATCGGTCACGGACC 3
R: 5-GGTATAACGGGTGCCATCACGG 3 | 58°C | S63418.1 | | CD117 | F: 5-TCCAAAACTCATCTGTCTCACC 3
R: 5-CCCACATCGTTATAAGCCCTG 3 | 58°C | AF263827.1 | | ACTB | F: 5-GGCCAACCGTGAGAAGATGACC 3
R: 5-GAGGCATACAGGGACAGCACAG 3 | 58°C | BT030480.1 | | UQCRB | F: 5-CCTTTATAATGACAGAGTGTTTCGC 3
R: 5-ACGGTTCAAGGTAGGATTTATCC 3 | 62°C | NM_001034797 | | ADA | F: 5-GAGGAGCTACAGAACATCATCG 3
R: 5-AATCCTTTTGACAGCCTCCC 3 | 62°C | NM_173887 | | F3 | F: 5-GAGTCCAGAAAGTCCCATCAAG 3
R: 5-TGATCACCAGCATCACTGTG 3 | 62°C | NM_173878 | | BPGM | F: 5-GTATTGCGTGGTAAAACCGTTC 3
R: 5-CCCCAGTAGGAAGAGTAATGTTG 3 | 62°C | NM_001035402 | | CFB | F: 5-GGGTGCTATTGTGTCTGAGTAC 3
R: 5-CTTTTACCTCCCACTCCTTCC 3 | 62°C | NM_001040526 | | SLC35A5 | F: 5-CTCAAGTCGCATCCTACTGG 3
R: 5-GACACAAGCGCACAGAAAAC 3 | 62°C | NM_001076025 | | SCRN1 | F: 5-ATCATGAACCTGAGAGCAAGG 3
R: 5-AGGTCTGCTTATCACAATGGC 3 | 62°C | NM_001110803 | | IGFBP3 | F: 5-CGCTACAAGGTTGACTACGAG 3
R: 5-GTTCAGCGTGTCTTCCATTTC 3 | 62°C | NM_174556 | | LOC781494 | F: 5-AAGATTCTGGAGAGTGCTGTG 3
R: 5-GACGAAGCAGATGGAGTACAC 3 | 62°C | NM_001101279 | | TFPI2 | F: 5-ACGTGTATGGACTTCTGTGC 3
R: 5-CCACACCCAGTATAGTTGAAGG 3 | 58°C | NM_182788 | | BAX2 | F: 5-AGGGTTTCATCCAGGATCGAGC 3
R: 5-TCATCTCCGATGCGCTTCAGAC 3 | 58°C | NM_001191220.1 | | COX2 | F: 5-GTCCCGTCCAGGCTTATATTAC 3
R: 5-GGACTAACTCAAGGACAATGGG 3 | 62°C | DQ347624.1 | | IL-1 | F: 5-AAGGAGAATGTGGTGATGGTG 3
R: 5-TGTAATGTGCTGATCTGGGC 3 | 62°C | NM_174092 |
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