Endometritis and In Vitro PGE₂ Challenge Modify Properties of Cattle Endometrial Mesenchymal Stem Cells and Their Transcriptomic Profile

Table 2

Clonal efficiency (CE) and cell doubling time (CDT) of fibroblast-like colony formation of cell derived from primary cultures in vitro of postpartum healthy endometrium (PPHE) with subclinical (PPSE) and clinical endometritis (PPCE). Different superscript letters in the same column indicate statistically significant differences. value < 0.05. Each experiment was repeated three times for each cell line.

Cell line	Number of colonies	CE (%)	CDT (hrs)
PPHE1	6.3	0.75	29.8
PPHE2	4.7	0.56	28.4
PPHE3	5.7	0.67	31.2
PPHE4	4.5	0.52	28.9
PPHE5	5.9	0.71	31.7
PPHE ( ± S.D.)	5.42 ± 0.7^a	0.64 ± 0.1^a	30 ± 0.4^a
PPSE1	4	0.48	33.9
PPSE2	4.3	0.52	35.2
PPSE3	3.7	0.45	34.2
PPSE4	3.9	0.55	34.9
PPSEl5	4.4	0.44	35.5
PPSE ( ± S.D.)	4.06 ± 0.3^ab	0.49 ± 0.1^ab	34.74 ± 0.7^ab
PPCE1	1.5	0.16	42
PPCE2	1	0.08	40.6
PPCE3	0	0	43.3
PPCE ( ± S.D.)	0.83 ± 0.8^b	0.08 ± 0.1^b	41.97 ± 1.4^b