Optogenetic Inhibition of Striatal Neuronal Activity Improves the Survival of Transplanted Neural Stem Cells and Neurological Outcomes after Ischemic Stroke in Mice

Figure 7

Excitation of GABAergic neurons can release factors that reduce NSC viability in vitro. (a) The schematic representation of the in vitro experiments. The blue arrows represent the stimulation with 473 nm laser pulse. (b) The viability of NSC, determined by CCK-8 assay at 2 hours (Con = 1.00 ± 0.03, Stim = 0.94 ± 0.02, ) and 12 hours (Con = 1.00 ± 0.01, Stim = 0.88 ± 0.02, ) after incubating with conditioned medium. (c) The relative mRNA expressions of NGF (Con = 1.00 ± 0.15, Stim = 0.37 ± 0.06, ). (d) The relative mRNA expressions of GDNF (Con = 1.00 ± 0.07, Stim = 0.54 ± 0.10, ). represents . represents .

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