Stem Cells International / 2017 / Article / Fig 4

Research Article

Regulation of Osteogenic Differentiation of Placental-Derived Mesenchymal Stem Cells by Insulin-Like Growth Factors and Low Oxygen Tension

Figure 4

PMSC multipotency and differentiation are regulated under low oxygen tension by IGFs. PMSCs were cultured for 14 days in osteogenic differentiation conditions containing 2% FBS in the presence or absence of 100 ng/mL of IGF-1 or IGF-2 in room air (20% O2) or low oxygen levels (1% O2). Treatments were stopped after 3, 7, and 14 days. Immunoblots, shown in Figure S, were used to quantify the changes in protein levels of (a) OCT4, (b) SOX2, (c) RUNX2, and (d) OPN induced by IGFs over time. Quantification levels were normalized to β-actin, a protein loading control (two-way ANOVA, , ). X indicates significance between different days without IGFs; # indicates significance of IGF addition compared with no IGFs in the same day.
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