Activin A Modulates CRIPTO-1/HNF4α⁺ Cells to Guide Cardiac Differentiation from Human Embryonic Stem Cells

Figure 6

CRIPTO-1 and HNF4α expression profiles in ActA treated human ESCs during early cardiac differentiation. HNF4α⁺ endodermal-like cells had a higher expression level of CRIPTO-1 in the control and 50 and 100 ng/mL ActA condition compared to the 10 and 25 ng/mL ActA treatment. Flow cytometry analyses of HNF4α and CRIPTO-1 expression after induction of human ESC-CM differentiation with the indicated concentrations of ActA. Representative example of three independent experiments.