Activin A Modulates CRIPTO-1/HNF4α⁺ Cells to Guide Cardiac Differentiation from Human Embryonic Stem Cells

Figure 7

Phenotyping CM differentiation showed both atrial- and ventricular-like (action potentials) APs. (a) Resting membrane potential (RMP) of human ESCs-CMs, measured during current clamp (whole-cell patch clamp recording) with or without a pretreatment of 100 ng/mL ActA. Each data point is a single cell measurement. (b) AP duration at 30% and 50% repolarization (APD30 and APD50). (c) Example of AP from a typical ventricular-like cell (left panel) and an atrial-like cell (right panel). (d) Percentages of CMs exhibiting an atrial-like or ventricular-like AP phenotype with and without ActA addition. (e) Simultaneous intracellular Ca²⁺ measurements using Fluo-4 and current clamp recording in a human ESC differentiated into CMs.

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