Human Suprapatellar Fat Pad-Derived Mesenchymal Stem Cells Induce Chondrogenesis and Cartilage Repair in a Model of Severe Osteoarthritis

Figure 4

Functional and structural cartilage regeneration. The therapeutic activity of suprapatellar-derived ASC (supra-hASC) in comparison with control-, SVF-, and PRP- treated groups was studied in a mouse model of severe OA. (a) Swelling-related inflammation was measured by quantifying once a week the external volume of the knees, versus control. (b) Glycosaminoglycan (GAG) quantification at both, tibia and femur, versus control. (c) Articular OA damage score was performed from 5 μm frontally sectioned knees stained with safranin-O and following the recommendations established by Glasson et al. [27]. versus control. Representative images of a frontal section stained with safranin-O are shown for each group. (d) Immunostaining of Sox9 (green) to evaluate the endogenous chondrogenic activity. All cell nuclei were detected with DAPI staining (blue).

(a)

(b)

(c)

(d)

(e)