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Stem Cells International
Volume 2017 (2017), Article ID 4794827, 9 pages
https://doi.org/10.1155/2017/4794827
Research Article

Characteristics of Human Endometrium-Derived Mesenchymal Stem Cells and Their Tropism to Endometriosis

1Department of Gynaecology, The First Affiliated Hospital of Harbin Medical University, Harbin, China
2Precision Medical Center, The Third Affiliated Hospital of Harbin Medical University, Harbin, China
3Department of Ultrasound of Obstetrics and Gynaecology, The First Affiliated Hospital of Harbin Medical University, Harbin, China
4The State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China
5College of Science, Harbin Engineering University, Harbin, China

Correspondence should be addressed to Guangmei Zhang; moc.621@gnahziemgnaug

Received 5 January 2017; Revised 28 March 2017; Accepted 21 May 2017; Published 6 July 2017

Academic Editor: James Adjaye

Copyright © 2017 Yan Cheng et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Human endometrial tissue has become an attractive source of mesenchymal stem cells (MSCs) for cell-based therapies because these MSCs can be easily harvested and have tumour tropism as well as reduced immunogenic and inflammatory properties. Our study aimed to obtain and characterise human endometrial mesenchymal stem cells (EMSCs) and assess their endometriosis tropism. EMSCs were successfully isolated from the endometrium of women undergoing laparoscopy for idiopathic infertility. The EMSCs presented a fibroblast-like morphology during culture. Flow cytometry analyses showed that the cells were positive for the specific stem cell markers CD73, CD90, CD105, CD166, and HLA-ABC (major histocompatibility complex class I (MHC I)) but negative for CD14, CD34, CD45, and HLA-DR (MHC II). Reverse transcription polymerase chain reaction results showed that the EMSCs expressed the stem cell marker OCT4. The EMSCs could differentiate into osteocytes, adipocytes, and chondrocytes under certain conditions. The EMSCs had a high tropism to endometriosis without tumourigenicity. This study enhances the possibility of using EMSCs as drug carriers in human cell-based therapies. Meanwhile, future research could also focus on developing targeted therapies for endometriosis.