Characteristics of Human Endometrium-Derived Mesenchymal Stem Cells and Their Tropism to Endometriosis

Figure 1

Isolation, cultivation, and identification of EMSCs. (a) (A-1) Nonadherent cells; (A-2) P1 cells; (A-3) P3 cells; (A-4) P5 cells. Scale bar = 1000 μm. (b) Stem cell-specific marker OCT4 expression in P3, P5, P10, P15, and P20 EMSCs. OCT4 was positively expressed in EMSCs, and the OCT4 expression shown a gradually decreasing trend with increasing passage number. (c) Immunophenotypes of EMSCs. The flow cytometry analysis results showed that the EMSCs positively expressed CD73, CD90, CD105, and CD166 but negatively expressed CD14, CD34, and CD45. The results also showed that the EMSCs expressed HLA-ABC (MHC I), but not HLA-DR (MHC II). (d) EMSC differentiation in vitro. The staining results of EMSCs after being cultured in different induction media.

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