Biological Characteristics of Fluorescent Superparamagnetic Iron Oxide Labeled Human Dental Pulp Stem Cells

Figure 7

MRI and histological examination of MIRB-labeled hDPSCs cell sheets/root fragments complex in vivo. (a) In vivo MR images of nude mice immediately after transplantation. (b) In vivo MR images of nude mice 30 days after transplantation. (c) In vivo MR images of nude mice 60 days after transplantation. Left boxed area indicates the unlabeled cell sheets (high signal image) and right boxed area indicates the labeled cell sheets. (d) Quantification analysis of the signal intensity of MIRB from the boxed area of (a), (b), and (c). . (e) Prussian blue staining of the MIRB-labeled group immediately after transplantation. (f) Prussian blue staining of the MIRB-labeled group 30 days after transplantation. (g) Prussian blue staining of the MIRB-labeled group 60 days after transplantation. (h) Prussian blue staining of the control group 60 days after transplantation. The scale bar of (e–h) indicates 500 μm.

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