Research Article
Adipose Tissue-Derived Mesenchymal Stem Cells Have a Heterogenic Cytokine Secretion Profile
Figure 3
Frequency of IL-6 and IFN-γ secreting ASC. ASC () were cultured without or in the presence of IFN-γ or TGF-β for 72 h and washed and reseeded at different cell densities. Frequencies of IL-6 (a) and IFN-γ (b) secreting ASC were determined by ELISPOT assay. Representative examples of the ELISPOT assay are shown. After TGF-β treatment, a higher frequency of IFN-γ producing ASC was found (, one-way ANOVA).
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