The Leukemic Stem Cell Niche: Adaptation to “Hypoxia” versus Oncogene Addiction

Figure 1

Suppression of BCR/Abl protein in CML cells undergoing “adaptation to hypoxia.” K562 cells (a), KCL22 cells (b), or blast-crisis primary cells (c) were plated at the indicated time-zero cell concentrations and incubated in atmosphere at 0.1% or 21% O₂. Cell lysates obtained at the indicated incubation times were immunoblotted using anti-c-Abl (detecting BCR/Abl) or anti-phospho-CRKL antibodies or, as loading equalization control, anti-GAPDH or anti-ERK1/2 antibodies; migration of molecular weight markers is indicated on the left (kDa). One out of three independent experiments with similar outcome is shown. Glucose concentration in the medium of cultures incubated at 0.1% O₂ was measured at the indicated incubation times as described in Materials and Methods. Values are means ± SD of data from 3 independent experiments; and versus time 0; ^# and ^## versus day 3; ^§ versus day 7.

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