Human Chorionic Plate-Derived Mesenchymal Stem Cells Restore Hepatic Lipid Metabolism in a Rat Model of Bile Duct Ligation

Figure 3

Expression of genes associated with fatty acid oxidation after BDL and/or CP-MSC transplantation. mRNA expression levels of PPARα (a) and CPT1A (b) by real-time PCR. β-Actin was used as an internal control for normalization. Data are expressed as a fold change related to the control group. (c) Protein expression levels of PPARα and CPT1A. GAPDH was used as a loading control, and quantification by densitometry of Western blots was normalized to GAPDH. Data are expressed as a fold change related to the control group. (d) Analysis of CPT1A expression with immunofluorescence staining (scale bar = 200 μm; original magnification, ×400). Liver tissues, which were collected at 3 weeks posttransplantation in the transplanted group and post-BDL in the nontransplanted group, were used in immunofluorescence staining. (e) mRNA expression levels of miR-33. U6 snRNA was used as an internal control for normalization. (compared to the nontransplanted group). CTL: control group; NTx: nontransplanted group; Tx: transplanted group.

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