Combined Analysis of Endothelial, Hematopoietic, and Mesenchymal Stem Cell Compartments Shows Simultaneous but Independent Effects of Age and Heart Disease

Table 2

Frequency of endothelial progenitor cells, hematopoietic stem cells, and mesenchymal stem cells in the sternal bone marrow samples from patients with ischemic or valvular heart disease, in presence or absence of left ventricular dysfunction. Cells were analyzed by colony assay (CFU) and flow cytometry (FACS).

Cell population (assay)	Heart disease			LV dysfunction
	IHD ()	VHD ()		Presence ()	Absence ()
EPCs (CFU)	1.9 (0.0–6.5)	0.6 (0.0–7.5)	0.675	1 (0–11)	18 (4–44)	0.038
EPCs (FACS)	174.1 ± 40.2	182.7 ± 51.7	0.611	171 ± 43.4	187 ± 45.6	0.341
HSCs (CFU)
BFU-E	6.5 (1–23)	1 (0–19)	0.411	21 (1–44)	9 (0–48)	0.727
CFU-GM	22 (6–46)	15.5 (2–46)	0.710	45 (9–95)	44 (7–94)	0.960
CFU-GEMM	54 (14–86)	18 (1–91)	0.313	67 (5–172)	76 (7–266)	0.881
HSCs (CFU)—total colonies	86 (22–154)	48 (5–144)	0.520	93 (14–309)	167 (13–322)	0.855
HSCs (FACS)	19.7 ± 6.6	17.6 ± 4.6	0.347	19.5 ± 6.3	17.9 ± 5.4	0.442
MSCs (CFU)	0.6 (0.3–0.9)	0.2 (0–0.7)	0.400	1 (1–1.5)	3 (1–6)	0.308

BFU-E: erythroid burst-forming units; CFU-GM: colony-forming units-granulocyte/macrophage: CFU-GEMM: multipotent myeloid stem cells; EPCs: endothelial progenitor cells; EV: ejection fraction; HSCs: hematopoietic stem cells; IHD: ischemic heart disease; LV: left ventricular; MSCs: mesenchymal stem cells; VHD: vascular heart disease. The results are presented as mean and standard deviation, or median and interquartile range. value, Student’s t-test and chi-square or Mann–Whitney test.