Evaluating Wharton’s Jelly-Derived Mesenchymal Stem Cell’s Survival, Migration, and Expression of Wound Repair Markers under Conditions of Ischemia-Like Stress
Effect of simulated ischemic stress on morphology of WJ-MSCs. Morphology of WJ-MSCs maintained under control conditions (a) and ischemic stress conditions mimicked by exposing WJ-MSCs to hypoxia (2% O2), low glucose, and serum-free medium for 48 hours (b). Representative phase contrast images from three independent biological samples of WJ-MSC () are presented. Comparison of total cell yield between control and ischemic WJ-MSCs. All cells were plated at a density of 5000 cells/cm2 and grown under control conditions for 72 hours. After 72 hours, control cultures were harvested while ischemic cultures were shifted to ischemia conditions for another 48 hours and then harvested () (c). Comparison of population doubling time between control and ischemic WJ-MSCs (d). Results represent the average of three independent cultures with SEM (Student’s -test, two tailed).