Research Article

Evaluating Wharton’s Jelly-Derived Mesenchymal Stem Cell’s Survival, Migration, and Expression of Wound Repair Markers under Conditions of Ischemia-Like Stress

Figure 5

Effect of long-term ischemia-like stress on morphology and viability of WJ-MSCs. The time course of exposure to ischemic conditions was extended to 5-6 days. Morphology of WJ-MSCs after being maintained under long-term ischemia (a). Flow cytometry analysis of apoptotic WJ-MSCs stained with annexin-V-PI following exposure to long-term ischemia (b). Senescence, as detected by senescence-associated β-galactosidase activity, in both long-term ischemia and control cultures (c). Quantification of the percentage of SA-β-gal positive blue cells of long-term ischemia and control WJ-MSC (d). Results are representative of at least three independent biological samples.