β-Arrestin1/miR-326 Transcription Unit Is Epigenetically Regulated in Neural Stem Cells Where It Controls Stemness and Growth Arrest

Figure 2

miR-326 and βarr-1 are upregulated in differentiated NSCs. (a) miR-326 levels evaluated in NSC exposed to differentiation stimuli (DFM) with retinoic acid (RA) or platelet-derived growth factor (PDGF) for 48 hours. Data are means ± SD from 3 independent experiments. . (b) Fluorescent in situ hybridization (ISH) staining of miR-326 of NSCs grown in stemness conditions (SM) and after RA-induced differentiation (DFM) for 48 hours. Nuclei are counterstained with Hoechst. Scale bar: 10 μm. Representative ISH images from 4 independent experiments. (c) mRNA expression levels of βarr-1 along with stemness and differentiation markers of NSC in SM and after differentiation (DFM). (d) Western blot (WB) analysis of endogenous βarr-1 and markers of stemness (Nanog, Sox2, and Oct4) and differentiation (βIII-tub and Gfap) of NSCs in SM and after differentiation (DFM). Gapdh as loading control (LC). ((c) and (d)) Data are means ± SD from 3 independent experiments. .

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