β-Arrestin1/miR-326 Transcription Unit Is Epigenetically Regulated in Neural Stem Cells Where It Controls Stemness and Growth Arrest

Figure 6

βarr-1 controls NSCs proliferation via p27 expression. (a) βarr-1 and p27 mRNA expression levels in NSCs transfected with the HA-βarr-1 plasmid for 48 hrs. (b) Subcellular localization of βarr-1 and p27 proteins in NSCs transfected with the HA-βarr-1 plasmid and analyzed 48 hrs after transfection. Proteins are shown in the cytosolic (Cyto) and nuclear (Nuc) fractions. Sp1: nuclear loading control; Gapdh: cytoplasmic loading control. Representative Western blot images from 3 independent experiments. (c) βarr-1 and p27 mRNA expression levels in NSCs transfected with control siRNA (siCtr) or βarr-1 siRNA (siβarr-1) cultured for 24 hrs in DFM. (d) Western blot showing endogenous βarr-1 and p27 protein levels in NSCs transfected with control siRNA (siCtr) or βarr-1 siRNA (siβarr-1) cultured for 24 hrs in DFM. Representative Western blot images from 3 independent experiments. ((a) and (c)) Data are means ± SD from 3 independent experiments. .

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