Stemness Maintenance Properties in Human Oral Stem Cells after Long-Term Passage

Figure 2

Mesengenic differentiation potential. hPDLSCs, hDPSCs, and hGMSCs induced to osteogenic commitment, stained with alizarin red S solution at P2 (a1, b1, and c1) and P15 (a2, b2, and c2) showed no statistical significative differences among two different culture stages. RUNX-2 and ALP expressions confirm light microscopy observations for osteogenic commitment (a3, b3, and c3). Adipogenic induction analyzed by oil red solution staining demonstrates the presence of lipid vacuoles at cytoplasmic level in cells cultured at early (d1, e1, and f1) and late passages (d2, e2, and f2). Both PPARγ and FABP4 showed no statistical differences after 28 days of culture, under differentiation conditions, at P2 and P15 (d3, e3, and f3). Mag.: 10x, bars: 10 μm.