Stemness Maintenance Properties in Human Oral Stem Cells after Long-Term Passage
Table 3
Cytofluorimetric analysis of hGMSCs.
Flow cytometry phenotyping of hGMSCs
Antigen
Phenotype
hGMSC MFI
P2
P15
value
CD13
+++
150.7 ± 40.4
299.7 ± 48.9
0.057
CD14
−
1.1 ± 0.2
1.3 ± 0.2
0.079
CD29
++
113.1 ± 20.2
106.8 ± 18.7
0.356
CD31
−
1.2 ± 0.3
1.4 ± 0.1
0.139
CD34
−
1.1 ± 0.2
1.2 ± 0.1
0.437
CD44
+++
163.9 ± 31.5
143.4 ± 33.9
0.245
CD45
−
1.2 ± 0.1
1.1 ± 0.1
0.230
CD73
+
29.2 ± 8.9
22.3 ± 5.9
0.171
CD90
+++
273.1 ± 58.9
156.7 ± 36.7
0.092
CD105
+
9.4 ± 1.7
5.8 ± 1.2
0.085
CD117
−
1.2 ± 0.2
1.3 ± 0.2
0.211
CD133
−
1.4 ± 0.3
1.2 ± 0.3
0.253
CD166
+
16.5 ± 2.5
20.9 ± 2.7
0.090
CD326
−
1.2 ± 0.2
1.1 ± 0.1
0.277
HLA-ABC
++
112.1 ± 35.4
86.6 ± 39.1
0.398
HLA-DR
−
1.3 ± 0.1
1.2 ± 0.1
0.403
NANOG
+
7.8 ± 2.5
6.0 ± 1.9
0.225
OCT4
+
4.3 ± 0.7
1.7 ± 0.5
↓0.019
SSEA4
+
3.4 ± 0.8
5.4 ± 1.4
0.148
SOX2
+
44.9 ± 6.8
45.1 ± 8.8
0.193
− indicates negative expression (0%); + indicates moderate expression; ++ indicates positive; +++ indicates high expression (100%); MFI ratio is the average of five different biological ; bold values represent MFI ratio with ; cutoff MFI ratio .
