CD60b: Enriching Neural Stem/Progenitor Cells from Rat Development into Adulthood

Figure 8

Characterization and differentiation of neurospheres derived from CD60b-positive cells. Immunostaining of neurospheres derived from CD60b-positive cells (a–c). As expected, most of the cells inside the neurosphere express nestin (green); a subpopulation of these cells also expresses CD60b (red). Some cells differentiated from neurospheres also expressed CD60b (b’). (d) Differentiation of neurospheres originated from CD60b-positive adult cells in GFAP-positive astrocytes (green) and TUJ1-positive neurons (red). Nuclei are stained with TO-PRO-3 in blue. Scale bar: 40 μm (a–c); 46 μm (b’); 35 μm (d).

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