Delayed Mesoderm and Erythroid Differentiation of Murine Embryonic Stem Cells in the Absence of the Transcriptional Regulator FUBP1

Figure 6

ESC/OP9 coculture in the presence of SCF and EPO for erythroid differentiation. The erythroid differentiation of Fubp1 KO ESCs was significantly reduced compared to NTC control ESCs, while the amount of CD71⁻/Ter119⁻ ESCs that were not differentiated into the erythroid lineage was increased in the Fubp1 KO clones (70% KO versus 59% NTC). Maturation of erythroid cells started with the expression of CD71 (CD71⁺/Ter119⁻ cells) in the proerythroblasts. CD71^high/Ter119⁻ cells: late erythroblasts; CD71⁺/Ter119⁺ cells: reticulocytes; CD71⁻/Ter119⁺: mature red blood cells. Three independent experiments were performed, each with 3 NTC and 6 Fubp1 knockout ESC clones. The data represent the mean values ± SD ( ; ).