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Stem Cells International
Volume 2017, Article ID 5762301, 12 pages
Research Article

Delayed Mesoderm and Erythroid Differentiation of Murine Embryonic Stem Cells in the Absence of the Transcriptional Regulator FUBP1

1Georg-Speyer-Haus, Institute for Tumor Biology and Experimental Therapy, Paul-Ehrlich-Strasse 42-44, 60596 Frankfurt/Main, Germany
2LOEWE Center for Cell and Gene Therapy Frankfurt and Department for Medicine, Hematology/Oncology, Goethe University Hospital Frankfurt/Main, 60590 Frankfurt/Main, Germany
3Institute of Biochemistry II, Goethe University Frankfurt, 60590 Frankfurt/Main, Germany
4German Cancer Consortium (DKTK), 69120 Heidelberg, Germany

Correspondence should be addressed to Martin Zörnig; ed.trufknarf-inu.hsg@ginreoz

Received 21 December 2016; Revised 2 March 2017; Accepted 19 March 2017; Published 15 May 2017

Academic Editor: Zhaohui Ye

Copyright © 2017 Josephine Wesely et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplementary Materials and Methods. Figure S1: CRISPR/Cas9-induced destruction of the Fubp1 open reading frame and creation of premature stop codons. Figure S2: Absence of FUBP1 does not affect ESC pluripotency according to SSEA1/SSEA4 marker expression. Figure S3: Cell cycle and FUBP1 target gene analysis in undifferentiated Fubp1 KO and NTC control ESC clones. Figure S4: Fubp1 mRNA expression in Flk-1+ cells. Figure S5: Differentiation of ESCs into CD45+ hematopoietic cells during OP9 coculture.

  1. Supplementary material