Isolation and Molecular Characterization of Amniotic Fluid-Derived Mesenchymal Stem Cells Obtained from Caesarean Sections

Figure 1

Amniotic fluid cell preparation. Amniotic fluid was collected during Caesarean sections using a 60 ml syringe (a). The AF was washed with PBS and centrifuged resulting in a reddish pellet (b, c). The red colour indicated the presence of erythrocytes which were then lysed using ammonium chloride at 4°C. The resulting white cell pellet (d, e) was transferred to cell culture vessels showing high level of heterogeneity (f). After, prolonged in vitro culture and passaging heterogeneous cell morphology (g) became uniform showing spindle-shaped fibroblast-like morphology (h).

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