Isolation and Molecular Characterization of Amniotic Fluid-Derived Mesenchymal Stem Cells Obtained from Caesarean Sections

<div>Protein expression analysis of AF-MSCs<i>.</i> (a) By immunofluorescent staining, AF-MSCs were found to express vimentin (A1), CD133 (A3), c-Kit (A4), SSEA4 (A7), TRA-1-60 (A8), and TRA-1-81 (A9) and by parallel absence of E-cadherin (A2), NANOG (A5), and OCT4 (A6). Cell nuclei were stained using Hoechst. (b) Flow cytometric analysis confirmed cell surface expression of SSEA4 (B1), TRA 1-60 (B2), and TRA-1-81 (B3).</div>

Stem Cells International

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Figure 3

Figure 3: Isolation and Molecular Characterization of Amniotic Fluid-Derived Mesenchymal Stem Cells Obtained from Caesarean Sections 