Isolation and Molecular Characterization of Amniotic Fluid-Derived Mesenchymal Stem Cells Obtained from Caesarean Sections

Figure 5

Overlapping and distinct gene expression in AF-MSCs. Dendrogram resulting from hierarchical clustering (a) of global gene expression profiles of AF-MSCs, fMSCs, and established pluripotent stem cells (H1, H9, and iPSCs). Transcriptomes of AF-MSC1 and AF-MSC2 cluster with fMSC while those of the H1, H9, and iPSCs cluster separately. The heatmap of 17 commonly up- or downregulated genes (b) shows similar gene expression of AF-MSCs and fMSCs. Venn diagram analysis revealed shared gene expression between fMSC, AF-MSCs, and pluripotent stem cells (c). Pearson’s correlation coefficient was calculated based on the transcription data (d). Each replicate was pairwise compared with each other replicate. A value of 1 indicates perfect linear correlation while a value of 0 implies no correlation. Pearson correlation analysis of transcriptome data revealed a high correlation (green) of both AF-MSC1 and AF-MSC2 with fMSCs but low correlation (red) with pluripotent cells.

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