Plasma Rich in Growth Factors Induces Cell Proliferation, Migration, Differentiation, and Cell Survival of Adipose-Derived Stem Cells

Figure 3

PRGF accelerates adipocyte, osteocyte, and chondrocyte in vitro differentiation of human ASCs. Human ASCs were induced to differentiate toward the three mesodermal lineages in the presence or absence of 2.5% PRGF; (a) adipogenesis: the presence of PRGF induced higher intracellular lipid content by Oil Red O staining; (b) osteogenesis: both calcium deposits visualized by Alizarin Red staining and the phalloidin (green: which indicates actin cytoskeleton growth in osteocytes) and Cx43 expression (red: a known marker of mature osteocytes) were improved in the presence of PRGF; (c) chondrogenesis: Alcian blue staining (marker of the proteoglycan aggrecan deposits) and immunostaining of Sox9 (green: marker of chondrogenesis and chondrocyte differentiation) were higher in the presence of PRGF. Scale bar: 50 μm. versus 0% PRGF.