Selective and Irreversible Induction of Necroptotic Cell Death in Lung Tumorspheres by Short-Term Exposure to Verapamil in Combination with Sorafenib

Figure 5

zVAD and Nec1 partially rescued the viability of cells. (a) Cells growing under PPSS for 8–10 days were incubated with the indicated concentrations of VP and SF alone or in the presence of zVAD or Nec1 for 24 h. Cell viability was measured by the MTT assay. (b) Cells growing as FTS for 14–16 days were incubated with the indicated concentrations of VP and SF alone or in the presence of zVAD or Nec1 for 24 h. Cell viability was measured by the CCK assay. Results (X ± SD) are representative of two independent experiments performed in sextuplicate. and indicate and , respectively (ANOVA).

(a)

(b)