Human Mesenchymal Stem/Stromal Cells from Umbilical Cord Blood and Placenta Exhibit Similar Capacities to Promote Expansion of Hematopoietic Progenitor Cells In Vitro

Figure 1

MSCs from UCB and PL increase proliferation of the population enriched in CD34⁺CD38⁻Lin⁻ cells. (a) Representative culture of CD34⁺CD38⁻Lin⁻ cells in the presence of cytokines (day 14): (A) without MSCs, (B) with BM-MSCs, (C) with UCB-MSCs, and (D) with PL-MSCs (magnification: 20x). (b) Kinetics of CD34⁺CD38⁻Lin⁻ cell proliferation in the presence of MSCs and in the absence (dotted lines) or presence (solid lines) of cytokines. Cocultures were prepared in the presence (A) or absence (B) of cell-cell contact (MSCs-HPCs). Control without MSCs (no vignette); BM-MSCs (square); UCB-MSCs (circle); and PL-MSCs (triangle). Data are shown as the means ± SD for the fold increases in cell number (BM-MSCs: ; UCB-MSCs: ; and PL-MSCs ). and ♦ indicate statistically significant differences, .

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