Effect of Uniaxial Tensile Cyclic Loading Regimes on Matrix Organization and Tenogenic Differentiation of Adipose-Derived Stem Cells Encapsulated within 3D Collagen Scaffolds

Table 1

Forward and reverse primers used for real-time PCR.

Gene	Forward primer	Reverse primer	Ref
GAPDH	5 AGAAGGCTGGGGCTGATTTG 3	5 AGGGCCCATCCACAGTCTTC 3	[33]
COL I	5 GGCTCCTGCTCCTCTTAGCG 3	5 CATGGTACCTGAGGCCGTTC 3	[25]
COL III	5 CAGCGGTTCTCCAGGCAAGG 3	5 CTCCAGTGATCCCAGCAATCC 3	[25]
DCN	5 CGCCTCATCTGAGGGAGCTT 3	5 TACTGGACCGGGTTGCTGAA 3	[25]
ACAN	5 CACTGTTACCGCCACTTCCC 3	5 ACCAGCGGAAGTCCCCTTCG 3	[34]
TCN	5 GGTGGATGGATTGTGTTCCTGAGA 3	5 CTGTGTCCTTGTCAAAGGTGGAGA 3	[25]
SCX	5 ACACCCAGCCCAAACAGA 3	5 GCGGTCCTTGCTCAACTTTC 3	[25]
TNMD	5 CCATGCTGGATGAGAGAGGT 3	5 CTCGTCCTCCTTGGTAGCAG 3	[34]
RUNX2	5 CAACCACAGAACCACAAGTGC 3	5 TGTTTGATGCCATAGTCCCTCC 3	[25]
ALP	5 GATCTTCTTTCTCCTTTGCCTGG 3	5 TGTTTGCAGTGGTGGTTCTGGCA 3	[26]
COL II	5 GGCAATAGCAGGTTCACGTACA 3	5 CGATAACAGTCTTGCCCCACTT 3	[35]
Sox 9	5 CACACAGCTCACTCGACCTTG 3	5 TTCGGTTATTTTTAGGATCATCTCG 3	[26]
MyoD	5 GCAGGTGTAACCGTAACC 3	5 ACGTACAAATTCCCTGTAGC 3	[36]
MYOG	5 GCCACAGATGCCACTACTTC 3	5 CAACTTCAGCACAGGAGACC 3	[36]