Predicting the Remaining Lifespan and Cultivation-Related Loss of Osteogenic Capacity of Bone Marrow Multipotential Stromal Cells Applicable across a Broad Donor Age Range

<div>Combination of<i> SPARC</i> and other parameters with predictive potential for MSC culture longevity. (a) No relationship between T/S ratio and PD-BS (left; solid: culture expanded, hollow: postosteogenic induction), but correlation between<i> SPARC</i> × [T/S ratio] and PD-BS (solid line: linear regression; dotted line: 95% confidence). (b) Left: 95% confidence in calculated values of<i> SPARC</i> × [1/PRAMEF2] in relation to PB-BS (shaded area ≤5 PD-BS); right: relationship between<i> SPARC</i> × [1/PRAMEF2] and CFU-O (c). Prediction model of calculation values &lt;5 being indicative of higher remaining PD-BS (left) and osteogenic potential (right). Whisker range 2.5%–97.5% confidence, line at median.</div>

Stem Cells International

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Figure 4

Figure 4: Predicting the Remaining Lifespan and Cultivation-Related Loss of Osteogenic Capacity of Bone Marrow Multipotential Stromal Cells Applicable across a Broad Donor Age Range 

Figure 4 | Predicting the Remaining Lifespan and Cultivation-Related Loss of Osteogenic Capacity of Bone Marrow Multipotential Stromal Cells Applicable across a Broad Donor Age Range 