Cytokine, Chemokine, and Growth Factor Profile Characterization of Undifferentiated and Osteoinduced Human Adipose-Derived Stem Cells

Figure 6

Calcium staining. Von Kossa staining was performed in order to show calcium deposition at day 14 for SaOs-2 and at day 21 for MG-63 and ASCs. In OM condition, the staining is more intense for each cell type. CM = control medium (DMEM 2% FBS); OM = osteogenic medium (see Materials and Methods). Images were taken at 100x magnification.