Molecular Mechanisms of Immunomodulation Properties of Mesenchymal Stromal Cells: A New Insight into the Role of ICAM-1

Figure 1

hASC suppress lymphocyte proliferation and downregulate IL-2Rα on activated T cells. Proliferation of PBMC activated with PHA and cultured separately and with hASC in contact or contactless conditions (25 : 1 ratio, 48 h) (a). CD25 expression in PBMC stimulated with plate-bound anti-CD3 and anti-CD28 and cultured with hASC (25 : 1, 48 h, mean + SEM, ). Culture medium and medium conditioned by hASC served as negative controls. Proportion and absolute number of CD25+ CD4 T cells determined using immunofluorescent staining and FACS analysis are shown (mean + SEM, ) (b, c). Similar analysis was performed using FACS sorted from PBMC pure (>95%) human CD4 T cells incubated with hASC (right plot); results obtained using total PBMC stained for CD25 are shown on the left plot; data of representative experiments are shown as histograms (d). Representative images of live PBMC-hASC cultures acquired using light microscope equipped with a digital camera. (48 h of incubation, magnification—10х) (e, f) (, , and ).

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