Molecular Mechanisms of Immunomodulation Properties of Mesenchymal Stromal Cells: A New Insight into the Role of ICAM-1

Figure 2

Increase in IDO level in hASC in the presence of activated PBMC. Supernatants from mixed cultures of activated PBMC (anti-CD3, anti-CD28) cultured with hASC (48 h) affect the CD25 level in CD4 T cells. After 48 hours of incubation, PBMC were analyzed for CD25 (IL-2Rα) surface expression (, mean + SEM) (a). Changes in IDO transcription after treatment of hASC with supernantants () (b). Transcriptional activity of IDO (c) and iNOS (d) genes in the course of hASC-mediated immune suppression (). IDO protein level in hASC cultured with anti-CD3- and anti-CD28-stimulated PBMC at 1 : 25 ratio for 48 hours (vinculin-normalization control) (e) ( and ; unstim—unstimulated PBMC, stim—PBMC stimulated with anti-CD3 and anti-CD28).

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