Molecular Mechanisms of Immunomodulation Properties of Mesenchymal Stromal Cells: A New Insight into the Role of ICAM-1

Figure 3

IDO inhibition blocks paracrine hASC-mediated immune suppression. Activated PBMC were cultured with or without hASC in contact and contactless conditions in the presence of 1-MT for 48 hours. Proportion of CD25+ CD4 T cells (a); IDO mRNA changes in hASC (b); IDO protein level in hASC cell lysates (c); kynurenine level in supernatants from hASC-PBMC cultures. Absorbance values (405 nm) were normalized to control samples (supernatant from hASC cultured alone). (d) All data represent mean + SEM of three independent experiments (1-MT—1-methyl-DL-tryptophan; and ).

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