Molecular Mechanisms of Immunomodulation Properties of Mesenchymal Stromal Cells: A New Insight into the Role of ICAM-1

Figure 7

ICAM-1 blocking inhibits hASC-mediated immunosuppression independently of IDO. CD3+ CD4 T cells sorted from PBMC and activated with anti-CD3/anti-СD28 were cultured alone or with hASC in the presence of anti-ICAM-1 or control IgG1 in contact or contactless conditions for 48 hours. Proportion and absolute numbers of CD25+ CD4 T cells were determined (a, b, resp.). hASC IDO gene expression (c); IDO protein level (d). Kynurenine level in mixed cultures (e) (mean + SEM, , and ). T cell-hASC cultures after control IgG1 (e) or anti-ICAM (f) treatment. Magnification 10x.

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