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Stem Cells International
Volume 2017, Article ID 6761572, 13 pages
Research Article

Age-Related Changes in Nucleus Pulposus Mesenchymal Stem Cells: An In Vitro Study in Rats

1Department of Orthopaedics, Navy General Hospital, Beijing, China
2Department of Orthopaedics, The 306th Hospital of People’s Liberation Army, Beijing, China
3Department of Laboratory Medicine, Children’s Hospital of Hebei Province, Shijiazhuang, Hebei, China

Correspondence should be addressed to Deli Wang; moc.361@yvaniledgnaw and Dike Ruan; moc.361@hgnekidnaur

Received 12 December 2016; Revised 23 January 2017; Accepted 6 February 2017; Published 15 March 2017

Academic Editor: Parinya Noisa

Copyright © 2017 Yachao Zhao et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The functions of mesenchymal stem cells (MSCs) appear to decline with age due to cellular senescence, which could reduce the efficacy of MSCs-based therapies. Recently, MSCs have been identified in the nucleus pulposus, which offers great potential for intervertebral disc (IVD) repair. However, this potential might be affected by the senescence of nucleus pulposus MSCs (NPMSCs), but whether or not this exists remains unknown. The aim of this study was to investigate the age-related changes in NPMSCs. NPMSCs isolated from young (3-month-old) and old (14-month-old) Sprague-Dawley rats were cultured in vitro. Differences in morphology, proliferation, colony formation, multilineage differentiation, cell cycle, and expression of β-galactosidase (SA-β-gal) and senescent markers (p53, p21, and p16) were compared between groups. Both young and old NPMSCs fulfilled the criteria for definition as MSCs. Moreover, young NPMSCs presented better proliferation, colony-forming, and multilineage differentiation capacities than old NPMSCs. Old NPMSCs displayed senescent features, including significantly increased G0/G1 phase arrest, increased SA-β-gal expression, decreased S phase entry, and significant p53-p21-pRB pathway activation. Therefore, this is the first study demonstrating that senescent NPMSCs accumulate in IVD with age. The efficacy of NPMSCs is compromised by donor age, which should be taken into consideration prior to clinical application.