Age-Related Changes in Nucleus Pulposus Mesenchymal Stem Cells: An In Vitro Study in Rats

Table 1

Primers utilized for real-time PCR amplification.

Target gene	Forward primer sequence	Reverse primer sequence
p53	5′-TACCGTATGAGCCACCTGAG-3′	5′-CAGGCACAAACACGAACCTC-3′
p21	5′-CAAAGTATGCCGTCGTCTGT-3′	5′-TCTCAGTGGCGAAGTCAAAG-3′
p16	5′-TACTCTCCTCCGCTGGGAAC-3′	5′-TGCCAGAAGTGAAGCCAAGG-3′
Collagen-II	5′-GGAAGAGTGGAGACTACTGGATTGAC-3′	5′-TCCATGTTGCAGAAAACCTTCA-3′
Aggrecan	5′-CCACTGGAGAGGACTGCGTAG-3′	5′-GGTCTGTGCAAGTGATTCGAG-3′
Sox-9	5′-AGGAAGCTGGCAGACCAGTACC-3′	5′-GGGTCTCTTCTCGCTCTCGTTCA-3′
β-Actin	5′-GGAGATTACTGCCCTGGCTCCTA-3′	5′-GACTCATCGTACTCCTGCTTGCTG-3′