Review Article
Safety of Cultivated Limbal Epithelial Stem Cell Transplantation for Human Corneal Regeneration
Table 1
Culture techniques of the included trial reports.
| | Author | Year | Eyes
transplanted (n) | Autologous/allogeneic | Scaffold | 3T3
(yes/no) | Serum | Duration of culture (days) | Animal-free culture conditions | GMP conditions |
| | Sangwan et al. [5] | 2006 | 78 | Autologous | HAM | No | FBS or AS | 10–14 | No | No | | Nakamura et al. [28] | 2006 | 9 | Allogenic (), autologous () | HAM | Yes (allo), no (auto) | AS | 15-16 | Yes | No | | Ang et al. [25] | 2007 | 1 | Allogenic | HAM | Yes | FS | Up to 28 | No | No | | Fatima et al. [6] | 2007 | 1 | Autologous | HAM | No | AS | Approx 14 | No | No | | Kawashima et al. [29] | 2007 | 6 | Autologous (), allogenic () | HAM | Yes | FS or AS | Approx 21 | No | No | | Shimazaki et al. [30] | 2007 | 27 | Autologous (), allogenic () | HAM | No (), yes () | AS | Approx 14–21 | No | No | | Shortt et al. [31] | 2008 | 10 | Autologous (), allogenic () | HAM | No | FS | 14–21 | No | Yes | | Satake et al. [7] | 2009 | 1 | Autologous | HAM | Yes | AS | 14 | No | No | | Di Girolamo et al. [8] | 2009 | 2 | Autologous | Silixane hydrogel CL | No | AS | 10 | Yes | No | | Meller et al. [26] | 2009 | 1 | Allogenic | HAM | No | AS | X | No | No | | Pauklin et al. [32] | 2010 | 44 | Autologous
(), allogenic
() | HAM | No | AS | Approx 14 | No | No | | Kolli et al. [9] | 2010 | 8 | Autologous | HAM | No | AS | 10–14 | Yes | Yes | | Gisoldi et al. [10] | 2010 | 6 | Autologous | Fibrin | Yes | X | 14–16 days | No | Yes | | Di Iorio et al. [11] | 2010 | 166 | Autologous | Fibrin | Yes | FBS | X | No | Yes | | Thanos et al. [12] | 2010 | 1 | Autologous | HAM | No | AS | X | No | No | | Rama et al. [13] | 2010 | 107 | Autologous | Fibrin | Yes | FBS | 14–16 | No | Yes | | Baradaran-Rafii et al. [14] | 2010 | 8 | Autologous | HAM (denuded) | No | FBS | 10–14 | No | No | | Sangwan et al. [15] | 2011 | 200 | Autologous | HAM | No | AS | 10–14 | Yes | No | | Sharma et al. [33] | 2011 | 50 | Autologous
(), allogenic
() | HAM | No | FBS | 21 | No | No | | Basu et al. [16] | 2012 | 50 | Autologous | HAM | No | AS | 10–14 | Yes | No | | Prabhasawat et al. [4] | 2012 | 19 | Autologous
(), allogenic
() | HAM denuded | No | AS | 15–23 | X | No | | Pellegrini et al. [24] | 2013 | 157 | Autologous | Fibrin | Yes | FBS | Approx 15 | No | Yes | | Sejpal et al. [17] | 2013 | 107 | Autologous | HAM | No | FBS or AS | 10–14 | No | No | | Pathak et al. [18] | 2013 | 9 | Autologous | HAM | No | AS | 14–21 | Yes | No | | Qi et al. [27] | 2013 | 42 | Allogenic | HAM | Yes | FBS | X | No | No | | Subramaniam et al. [19] | 2013 | 40 | Autologous | HAM | No | AS | 10–15 | No | No | | Sharma et al. [20] | 2013 | 4 | Autologous | HAM | No | FBS | 14 | No | No | | Vazirani et al. [21] | 2014 | 70 | Autologous | HAM | No | FBS or AS | 10–14 | No | No | | Zakaria et al. [35] | 2014 | 18 | Autologous
(), allogenic
() | HAM | No | AS | 14 | Yes | Yes | | Ramírez et al. [36] | 2015 | 20 | Autologous
(), allogenic
() | HAM | No | FBS | 7–14 | Yes | Yes | | Bobba et al. [22] | 2015 | 7 | Autologous | Silixane hydrogel CL | No | AS | 9–16 | Yes | No | | Pedrotti et al. [23] | 2015 | 13 | Autologous | Fibrin | Yes | FBS | Approx 14 | No | No |
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AS: autologous serum; FBS: fetal bovine serum; CL: contact lens; HAM: human amniotic membrane; 3T3: 3T3 feeder layer; GMP: good manufacturing practice; Approx: approximately.
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