BMP4/LIF or RA/Forskolin Suppresses the Proliferation of Neural Stem Cells Derived from Adult Monkey Brain

Figure 3

Characteristics of the neurospheres and neural stem cells. (a) Cells expressed neural stem cell marker protein Sox2. (b) Cells expressed neural stem cell maker protein Nestin. (c) Sphere growth pattern showed in yellow circles. Sphere core was slowly growing during the progress of sphere formation (SF), sphere adherence (SA), clone formation (CF), and clone extension (CE) in neural stem cell selective media with factors. (d) Cells proliferated from the spheres and adherent cell increased. Cells climbed from the spheres, and cover area of adherent cell pattern was showed in a big yellow border line. Yellow circles present the spheres. (e) Sphere size slightly seemed to be a downward trend but there was no significant difference. value = 0.461. (f) Cell growth area from sphere was more than a thousand times versus sphere. Scale bar = 25 μm. Data were shown as mean ± SD; value < 0.001.

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