BMP4/LIF or RA/Forskolin Suppresses the Proliferation of Neural Stem Cells Derived from Adult Monkey Brain

Figure 4

BMP4 inhibited cell proliferation and effected cell morphology. (a) Monkey NSCs treated in control media or BMP4 media for 6 days. (b) Cell morphology displayed the different phenotypes after BMP4 treatment under enlarged view. (c) Ki67-positive cells greatly reduced after BMP treatment compared with the control group. Ki67 was observed by immunofluorescence staining. (d) Cell amount presented that BMP4 suppressed the proliferation of monkey NSC-like cells. (e) The increase of cell adherent area showed that single cell became large after BMP4 treatment. (f) The length-width ratio suggested the cells became oblate from leptosomic type after BMP treatment. (g) Cells appeared shorter than control after BMP treatment. (a) and (b) Scale bar = 50 μm; (c) scale bar = 25 μm. Data represent the mean values ± SD; value < 0.001.

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