Research Article

Successful Low-Cost Scaffold-Free Cartilage Tissue Engineering Using Human Cartilage Progenitor Cell Spheroids Formed by Micromolded Nonadhesive Hydrogel

Figure 1

Scheme of cartilage progenitor cell spheroid culture. (a) Silicone molds were used from MicroTissues 3D Petri Dish. (b) The agarose solution was dispensed into silicone molds, resulting in the micromolded nonadhesive agarose hydrogel (c). (d) The cell suspension was carefully seeded into cell seeding chamber with single pipetting. (e) The cell suspension decanted into circular recesses after few minutes resulting in spheroids after 18 hours (f).
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