Tissue Source and Cell Expansion Condition Influence Phenotypic Changes of Adipose-Derived Stem Cells
Early and late markers of osteogenesis. RNA was isolated from cells differentiated in media supplemented with 10% FBS or 10% hPL, and expression of early and late markers was measured by RT-PCR and is presented for differentiated commercial ASCs (RB-OSTEO), undifferentiated controls (HAP-CTRL and BH-CTRL), and cells cultured under osteogenic conditions (HAP-OSTEO and BH-OSTEO). All conditions were normalized to undifferentiated controls (data not shown). Sp7 and Spp1 expression is given for FBS (a) and hPL (b) conditions at day 7. Alpl, Bglap, and Runx2 are presented for day 14 for FBS (c) and hPL (d). Statistically significant differences were determined by one-way ANOVA with Tukey’s multiple comparisons. Data represent the mean ± SEM of three biological replicates from three donors/group. RB represents the mean ± SEM of three biological replicates from one donor from a commercial source. Significant differences between groups are designated by alphabetical letters. Any groups with the same letter are not statistically different from each other (, Tukey’s MCT). A designation of indicates that transcript was not detected for this sample.
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